Bethyl Laboratories
Rabbit anti-NUP107 Antibody Affinity Purified
Primary
Catalog #A301-787A
TargetNUP107
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. About 30 different human Nups have been identified and appear to serve non-redundant functions. Nup107 is essential for the assembly of peripheral proteins in the NPC. It has also been observed to localize to kinetochores in a subcomplex with NUP160 and is essential for proper mitosis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 875 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetNUP107
Antigen Species
Human
Gene ID57122
Gene Symbol
NUP107
Gene Name
nucleoporin 107
Uniprot IDP57740
Protein Name
Nuclear pore complex protein Nup107
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NUP107 immobilized on solid support.
The epitope recognized by A301-787A maps to a region between residue 875 and 925 of human nucleoporin 107kDa using the numbering given in entry NP_065134.1 (GeneID 57122).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-787A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-787A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-787AOpen
Citations / Publications
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