Bethyl Laboratories
Rabbit anti-NUP133 Antibody Affinity Purified
Primary
Catalog #A302-386A
TargetNUP133
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 1
local_shipping
Order within 10 hours and 20 minutes for shipment by Tuesday, November 10
Product Description
The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. About 30 different human Nups have been identified and appear to serve non-redundant functions. Nucleoporin 133 kDa (NUP133) has been shown to play a role in mRNA export.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1106 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetNUP133
Antigen Species
Human
Gene ID55746
Gene Symbol
NUP133
Gene Name
nucleoporin 133
Uniprot IDQ8WUM0
Protein Name
Nuclear pore complex protein Nup133
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NUP133 immobilized on solid support.
The epitope recognized by A302-386A maps to a region between residue 1106 to 1156 of human nucleoporin 133kDa using the numbering given in entry NP_060700.2 (GeneID 55746).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-386A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-386A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-386AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1