Bethyl Laboratories
Rabbit anti-NUP153 Antibody Affinity Purified
Primary
Catalog #A301-788A
TargetNUP153
HostRabbit
ClonalityPolyclonal
ApplicationIP, WB
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Product Description
The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. About 30 different human Nups have been identified and appear to serve non-redundant functions. Nup153 contains four RanBP2-type zinc fingers and an RNA binding domain. The zinc-fingers of Nup153 can bind the small GTPase, Ran. The RNA binding domain has been shown to have an affinity for single-stranded RNA.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 50 and 100
Isotype
IgG
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetNUP153
Antigen Species
Human
Gene ID9972
Gene Symbol
NUP153
Gene Name
nucleoporin 153
Uniprot IDP49790
Protein Name
Nuclear pore complex protein Nup153
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NUP153 immobilized on solid support.
The epitope recognized by A301-788A maps to a region between residue 50 and 100 of human nucleoporin 153kDa using the numbering given in entry NP_005115.2 (GeneID 9972).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A301-788A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-788A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-788AOpen
Citations / Publications
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