Bethyl Laboratories
Rabbit anti-NUP160 Antibody Affinity Purified
Primary
Catalog #A301-791A
TargetNUP160
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. About 30 different human Nups have been identified and appear to serve non-redundant functions. Nup160 forms a subcomplex with Nup107. This subcomplex has been found to be important to bipolar spindle assembly and kinetochore function.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1100 and 1150
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNUP160
Antigen Species
Human
Gene ID23279
Gene Symbol
NUP160
Gene Name
nucleoporin 160
Uniprot IDQ12769
Protein Name
Nuclear pore complex protein Nup160
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NUP160 immobilized on solid support.
The epitope recognized by A301-791A maps to a region between residue1050 and 1100 of human nucleoporin 160kDa using the numbering given in entry BAA12110.1 (GeneID 23279).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A301-791A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-791A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-791AOpen
Citations / Publications
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