Bethyl Laboratories
Rabbit anti-NUP210 Antibody Affinity Purified
Primary
Catalog #A301-795A
TargetNUP210
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. About 30 different human Nups have been identified and appear to serve non-redundant functions. Nup210 is a membrane-spanning glycoprotein and major component of the NPC. It is essential for nuclear envelope structure and cell viability. Autoantibodies to Nup210 have been observed in patients with primary biliary cirrhosis (PBC), an autoimmune liver disease.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1837 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetNUP210
Antigen Species
Human
Gene ID23225
Gene Symbol
NUP210
Gene Name
nucleoporin 210
Uniprot IDQ8TEM1
Protein Name
Nuclear pore membrane glycoprotein 210
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NUP210 immobilized on solid support.
The epitope recognized by A301-795A maps to a region between residue 1837 and 1887 of human nucleoporin 210kDa using the numbering given in entry NP_079199.2 (GeneID 23225).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-795A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-795A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-795AOpen
Citations / Publications
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