Bethyl Laboratories
Rabbit anti-NUP50 Antibody Affinity Purified
Primary
Catalog #A301-782A
TargetNUP50
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. There are about 30 different human Nups that have been identified. Nup50 (also called Npap60) appears to not be a fixed structural component of the NPC. Nup50 has been shown to be a component of the importin complex, a protein carrier complex that imports cargo from the cytoplasm to the nucleus through the NPC. In this complex, Nup50 appears to have importance in the late stages of import and functions in complex disassembly and importin recycling.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 225 and 275
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNUP50
Antigen Species
Human
Gene ID10762
Gene Symbol
NUP50
Gene Name
nucleoporin 50
Uniprot IDQ9UKX7
Protein Name
Nuclear pore complex protein Nup50
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NUP50 immobilized on solid support.
The epitope recognized by A301-782A maps to a region between residue 225 and 275 of human nucleoporin 50kDa using the numbering given in entry NP_009103.2 (GeneID 10762).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-782A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-782A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-782A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-782AOpen
Citations / Publications
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