Bethyl Laboratories
Rabbit anti-NUP93 Antibody Affinity Purified
Primary
Catalog #A303-979A
TargetNUP93
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Nucleoporin 93 (NUP93) is a component of the p62 complex, a complex composed of NUP62 and NUP54. NUP93 forms a complex with NUP53, NUP155, NUP205 and lamin B; the interaction with NUP53 is direct [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8N1F7].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 575 and 625
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNUP93
Antigen Species
Human
Gene ID9688
Gene Symbol
NUP93
Gene Name
nucleoporin 93
Uniprot IDQ8N1F7
Protein Name
Nuclear pore complex protein Nup93
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NUP93 immobilized on solid support.
The epitope recognized by A303-979A maps to a region between residue 606 to 656 of human Nucleoporin 85kDa using the numbering given in entry NP_079120.1 (GeneID 9688).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-979A · Lot 2 (current lot)Open
Product DatasheetPDF · A303-979A · Lot 2 (current lot)Open
Safety Data SheetPDF · A303-979AOpen
Citations / Publications
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