Bethyl Laboratories

Rabbit anti-OLA1 Antibody Affinity Purified

Primary
Catalog #A305-459A
TargetOLA1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationWB
Detection of human and mouse OLA1 by western blot.
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Product Description

Obg-like ATPase 1 (OLA1) hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. OLA1 has lower affinity for GTP [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NTK5].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 50 and 100
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetOLA1
Antigen Species
Human
Gene ID29789
Gene Symbol
OLA1
Gene Name
Obg like ATPase 1
Uniprot IDQ9NTK5
Protein Name
Obg-like ATPase 1
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to OLA1 immobilized on solid support.

The epitope recognized by A305-459A maps to a region between residue 50 to 100 of human Obg-like ATPase 1 using the numbering given in entry Q9NTK5.2 (GeneID 29789).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western Blot (WB)1:2000 - 1:10,000

Documents

Product DatasheetPDF · A305-459A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-459A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-459AOpen
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