Bethyl Laboratories
Rabbit anti-ORC1 Antibody Affinity Purified
Primary
Catalog #A301-892A
TargetORC1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 1
local_shipping
Order within 18 hours and 32 minutes for shipment by today
Product Description
ORC1 (origin recognition complex, subunit 1) is one of six subunits that make up the origin of recognition complex (ORC). The ORC serves as a scaffold for proteins involved in the initiation of DNA replication. Very recently, ORC1 has been found to perform a function independent of its role in DNA replication. In a screen using siRNAs for human ORC proteins to assess their roles in centrosome biology, it was found that ORC1 plays a key role in controlling centriole and centrosome copy number in human cells. ORC1 is also known as replication control protein 1, ORC1L, HSORC1, and PARC1.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 811 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetORC1
Antigen Species
Human
Gene ID4998
Gene Symbol
ORC1
Gene Name
origin recognition complex subunit 1
Uniprot IDQ13415
Protein Name
Origin recognition complex subunit 1
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ORC1 immobilized on solid support.
The epitope recognized by A301-892A maps to a region between residue 811 and 861 of human origin recognition complex subunit 1 using the numbering given in entry NP_004144.2 (GeneID 4998).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-892A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-892A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-892AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1