Bethyl Laboratories
Rabbit anti-ORC2 Antibody Affinity Purified
Primary
Catalog #A302-735A
TargetORC2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. ORC2 is also known as ORC2L and is a subunit of the human ORC complex. ORC2 forms a core complex with ORC3L, -4L, and -5L [taken from NCBI Entrez Gene (GeneID: 4999)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetORC2
Antigen Species
Human
Gene ID4999
Gene Symbol
ORC2
Gene Name
origin recognition complex subunit 2
Uniprot IDQ13416
Protein Name
Origin recognition complex subunit 2
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ORC2 immobilized on solid support.
The epitope recognized by A302-735A maps to a region between residue 150 and 200 of human Origin Recognition Complex, Subunit 2-like using the numbering given in entry NP_006181.1 (GeneID 4999).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-735A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-735A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-735AOpen
Citations / Publications
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