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Rabbit anti-OTUB1 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
OTUB1
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 40 and 80
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Mouse, Human
Presumed Reactivity
Rat
Antigen Species
Human
Stock Concentration
40 µg/ml
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to OTUB1 immobilized on solid support.

The epitope recognized by A302-918A-T maps to a region between residue 40 and 80 of human OTU Domain, Ubiquitin Aldehyde Binding 1 using the numbering given in entry NP_060140.2 (GeneID 55611).

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Gene & Protein Information

OTUB1 (OTU domain, ubiquitin aldehyde binding 1) is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth [taken from NCBI Entrez Gene (Gene ID: 55611].

55611

OTUB1

OTU deubiquitinase, ubiquitin aldehyde binding 1

Q96FW1

Ubiquitin thioesterase OTUB1

Alternate Names

deubiquitinating enzyme OTUB1, hOTU1, HSPC263, OTB1, OTU domain, ubiquitin aldehyde binding 1, OTU domain-containing ubiquitin aldehyde-binding protein 1, OTU1, otubain-1, OTU-domain Ubal-binding 1, ubiquitin thioesterase OTUB1, ubiquitin-specific protease otubain 1, ubiquitin-specific-processing protease OTUB1

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-20% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.