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Primary Antibodies

Rabbit anti-OTULIN Antibody Affinity Purified

Name: Rabbit anti-OTULIN Antibody
Brand: Bethyl Laboratories
SKU: A305-822A
Price: 121 USD

Bethyl Laboratories

Catalog #

Target:

OTULIN

Reactivity:

Human

Applications:

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 302 and 352

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications

Verified Reactivity

Human

Antigen Species

Human

Concentration

1000 µg/ml

Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State

Liquid

Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Request Formulation Change

Additional Product Information

Otulin is a deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex. OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent proinflammatory signaling. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96BN8].

Gene ID:

90268

Gene Symbol:

OTULIN

Gene Name:

OTU deubiquitinase with linear linkage specificity

Uniprot ID:

Q96BN8

Protein Name:

Ubiquitin thioesterase otulin

Alternate Names

AIPDS; deubiquitinating enzyme otulin; FAM105B; family with sequence similarity 105, member B; GUM; OTU domain-containing deubiquitinase with linear linkage specificity; ubiquitin thioesterase Gumby; ubiquitin thioesterase otulin

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.