Bethyl Laboratories
Rabbit anti-p14ARF Antibody Affinity Purified
Primary
Catalog #A300-342A
Targetp14ARF
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
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Product Description
p16INK4a is a negative regulator of CDK4 and CDK6 kinase activity and cell cycle progression. p16INK4a regulates the cell cycle by inhibiting cyclin/CDK complex formation and the phosphorylation of the retinoblastoma protein (pRb). The gene which encodes p16INK4a, CDKN2A, is frequently mutated or deleted in a wide variety of tumors and is known to be an important tumor suppressor gene. The CDKN2A gene also generates a distinct transcript variant and protein, p14ARF, from an alternate open reading frame (ARF).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 82 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
Targetp14ARF
Antigen Species
Human
Gene ID1029
Gene Symbol
CDKN2A
Gene Name
cyclin dependent kinase inhibitor 2A
Uniprot IDP42771
Protein Name
Cyclin-dependent kinase inhibitor 2A
Alternate Names
alternative reading frame • ARF • CDK4 inhibitor p16-INK4 • CDK4I • CDKN2 • cell cycle negative regulator beta • CMM2 • cyclin-dependent kinase 4 inhibitor A • cyclin-dependent kinase inhibitor 2A • cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) • INK4 • INK4A • MLM • MTS1 • MTS-1 • multiple tumor suppressor 1 • P14 • P14ARF • P16 • P16INK4 • p16-INK4 • P16INK4A • P16-INK4A • P19 • P19ARF • TP16
Product Information
Applications
IHC, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to p14ARF immobilized on solid support.
The epitope recognized by A300-342A maps to a region between residue 82 and the C-terminus (residue 132) of human p14ARF using the numbering given in entry NP_478102.2 (GeneID 1029).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:500 - 1:2500
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Documents
Product DatasheetPDF · A300-342A · Lot 3 (current lot)Open
Product DatasheetPDF · A300-342A · Lot 3 (current lot)Open
Safety Data SheetPDF · A300-342AOpen
Citations / Publications
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