/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-p21/CIP1/CDKN1A Recombinant Monoclonal Antibody [BLR337M]
Primary Antibodies  

Rabbit anti-p21/CIP1/CDKN1A Recombinant Monoclonal Antibody [BLR337M]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

p21/CIP1/CDKN1A

Reactivity:

Human

Applications:

ICC

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR337M

Format:

Whole IgG

Immunogen:

between 100 and 150

Isotype:

IgG

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

$127.00 $794.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
500 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a peptide representing a region between residue 100 and 150 of human cyclin-dependent kinase inhibitor 1 (aka p21 or CIP1) using the numbering given in entry NP_000380.1 (Gene ID 1026).

Additional Product Information

p21/CIP1/CDKN1A is a potent cyclin-dependent kinase inhibitor. It binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression of p21/CIP1/CDKN1A is tightly controlled by the tumor suppressor protein p53, and it mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. p21/CIP1/CDKN1A can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. [taken from NCBI Entrez Gene (Gene ID: 1026)].

1026

CDKN1A

cyclin dependent kinase inhibitor 1A

P38936

Cyclin-dependent kinase inhibitor 1

Alternate Names

CAP20;CDK-interacting protein 1;CDK-interaction protein 1;CDKN1;CIP1;cyclin-dependent kinase inhibitor 1;cyclin-dependent kinase inhibitor 1A (p21, Cip1);DNA synthesis inhibitor;MDA-6;melanoma differentiation associated protein 6;Melanoma differentiation-associated protein 6;P21;p21CIP1;SDI1;WAF1;wild-type p53-activated fragment 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.