Bethyl Laboratories
Rabbit anti-p600 Antibody Affinity Purified
Primary
Catalog #A302-279A
Targetp600
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
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Product Description
p600 is an E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, p600 forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Additionally p600 regulates integrin-mediated signaling and may play a role in activation of FAK in response to cell-matrix interactions [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5T4S7].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 5133 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
Targetp600
Antigen Species
Human
Gene ID23352
Gene Symbol
UBR4
Gene Name
ubiquitin protein ligase E3 component n-recognin 4
Uniprot IDQ5T4S7
Protein Name
E3 ubiquitin-protein ligase UBR4
Alternate Names
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to p600 immobilized on solid support.
The epitope recognized by A302-279A maps to a region between residue 5133 and 5183 of human retinoblastoma-associated factor 600 using the numbering given in entry NP_065816.1 (GeneID 23352).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000 - 1:5000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A302-279A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-279A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-279AOpen
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