Bethyl Laboratories
Rabbit anti-p66alpha IHC Antibody Affinity Purified
Primary
Catalog #IHC-00669
Targetp66alpha
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
p66alpha and p66beta are human orthologs of the xenopus p66 subunit of the Mi-2/NuRD complex. The Mi-2/NuRD complex is a multi-subunit complex that mediates gene repression via processes involving chromatin remodeling and histone deacetylation. p66 alpha and beta were identified in a two-hybrid screen using the methyl-CpG-binding domain protein, MBD2, as bait. It has been demonstrated that p66alpha and p66beta mediate the interaction between MBD2 and histones.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 583 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
Targetp66alpha
Antigen Species
Human
Gene ID54815
Gene Symbol
GATAD2A
Gene Name
GATA zinc finger domain containing 2A
Uniprot IDQ86YP4
Protein Name
Transcriptional repressor p66-alpha
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to p66alpha immobilized on solid support.
The epitope recognized by IHC-00669 maps to a region between residue 573 and 633 of human p66alpha using the numbering given in entry AAO31797.1 (GeneID 54815).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00669Open
Product DatasheetPDF · IHC-00669 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00669 · Lot 1 (current lot)Open
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