Bethyl Laboratories
Rabbit anti-p66beta/GATAD2B Antibody Affinity Purified
Primary
Catalog #A301-283A
Targetp66beta/GATAD2B
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
P66beta/GATAD2B interacts with MBD2 (methyl-CpG-binding domain 2) and MBD3 (methyl-CpG-binding domain 3) and participates in transcriptional repression in association with the MBD2/NuRD and MBD3/NuRD histone deacetylase complexes. P66beta/GATAD2B is SUMOylated in vivo and its repressive activity has been shown to be enhanced by SUMOylation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 543 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
Targetp66beta/GATAD2B
Antigen Species
Human
Gene ID57459
Gene Symbol
GATAD2B
Gene Name
GATA zinc finger domain containing 2B
Uniprot IDQ8WXI9
Protein Name
Transcriptional repressor p66-beta
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to p66beta/GATAD2B immobilized on solid support.
The epitope recognized by A301-283A maps to a region between residue 543 and 593 of human transcription repressor p66 beta component of the MeCP1 complex (GATA zinc finger domain containing 2B ) using the numbering given in entry NP_065750.1 (GeneID 57459).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-283A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-283A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-283AOpen
Citations / Publications
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