Bethyl Laboratories
Rabbit anti-PAF49 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00474
TargetPAF49
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF, IHC
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Product Description
PAF49 is subunit of RNA polymerase I. PAF49/CAST has been shown to interact with activator upstream binding factor (UBF) and plays an important role in rDNA transcription.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 425 and 475
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetPAF49
Antigen Species
Human
Gene ID10849
Gene Symbol
CD3EAP
Gene Name
CD3e molecule associated protein
Uniprot IDO15446
Protein Name
DNA-directed RNA polymerase I subunit RPA34
Alternate Names
A34.5 • antisense to ERCC-1 protein • ASE1 • ASE-1 • CAST • CD3e antigen, epsilon polypeptide associated protein • CD3e molecule, epsilon associated protein • CD3E-associated protein • CD3-epsilon-associated protein • DNA-directed RNA polymerase I subunit RPA34 • PAF49 • RNA polymerase I-associated factor PAF49
Product Information
Applications
ICC-IF, IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PAF49 immobilized on solid support.
The epitope recognized by IHC-00474 maps to a region between residue 404 and 454 of human RNA polymerase I-associated factor PAF49 (CD3-epsilon-associated protein) using the numbering given in entry NP_036231.1 (GeneID 10849).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500
Immunohistochemistry (IHC)1:100 - 1:1,500
Documents
Safety Data SheetPDF · IHC-00474Open
Product DatasheetPDF · IHC-00474 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00474 · Lot 1 (current lot)Open
Citations / Publications
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