Bethyl Laboratories
Rabbit anti-PAK1 Antibody Affinity Purified
Primary
Catalog #A301-259A
TargetPAK1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
PAK1 (p21-activated kinase 1) is part of the family of PAK serine/threonine p21-activating protein kinases involved in cell survival, migration, and invasion. PAK1 mediates signals from extracellular stimuli to the cytoplasm and nucleus via its association with the small GTP-binding proteins, Cdc42 and Rac. GTPase binding to PAK1 activates PAK1 kinase activity and leads to reorganization of the actin cytoskeleton and the phosphorylation of proteins involved in survival, energy metabolism, gene expression, cell motility, cell morphology, and the cell cycle.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetPAK1
Antigen Species
Human
Gene ID5058
Gene Symbol
PAK1
Gene Name
p21 (RAC1) activated kinase 1
Uniprot IDQ13153
Protein Name
Serine/threonine-protein kinase PAK 1
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PAK1 immobilized on solid support.
The epitope recognized by A301-259A maps to a region between residue 1 and 50 of human p21/Cdc42/Rac1-activated kinase 1 using the numbering given in entry NP_002567.3 (GeneID 5058).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-259A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-259A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-259AOpen
Citations / Publications
Services
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