Rabbit anti-PAM16 Antibody Affinity Purified

Specifications

Production & Epitope

Antibody was affinity purified using an epitope specific to PAM16 immobilized on solid support.

The epitope recognized by A305-699A maps to a region between residue 75 to 125 of human Mitochondrial import inner membrane translocase subunit TIM16 using the numbering given in entry Q9Y3D7.2 (GeneID 51025).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.