Bethyl Laboratories
Rabbit anti-PAPOLA Antibody Affinity Purified
Primary
Catalog #A301-009A
TargetPAPOLA
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Poly(A) polymerase alpha (PAPOLA) is the polymerase responsible for catalyzing the addition of adenosine to the 3’end of pre-mRNAs for the synthesis of the 3’-end poly(A) tail. PAPOLA associates with the cleavage and polyadenylation specificity factor (CPSF) and Fip1 to perform this function.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 600 and 650
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPAPOLA
Antigen Species
Human
Gene ID10914
Gene Symbol
PAPOLA
Gene Name
poly(A) polymerase alpha
Uniprot IDP51003
Protein Name
Poly(A) polymerase alpha
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PAPOLA immobilized on solid support.
The epitope recognized by A301-009A maps to a region between residue 600 and 650 of human poly(A) polymerase alpha using the numbering given in entry NP_116021.2 (GeneID 10914).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-009A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-009A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-009AOpen
Citations / Publications
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