Bethyl Laboratories
Rabbit anti-PARP1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00279
TargetPARP1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
PARP1 (Poly-ADP-ribose polymerase 1) is a member of PARP family of enzymes that transfer the ADP-D-ribosyl group of NAD(+) to an acceptor carboxyl group on proteins. PARP1 catalyzes the poly-ADP-ribosylation of histone and non-histone proteins in response to DNA damage. The over-activation of PARP-1 in response to DNA damage has been shown to promote cell and tissue injury. This observation has initiated research into the therapeutic potential of PARP1 inhibitors in the treatment of cancer, cardiovascular disease, and brain injury.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 50 and 100
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetPARP1
Antigen Species
Human
Gene ID142
Gene Symbol
PARP1
Gene Name
poly(ADP-ribose) polymerase 1
Uniprot IDP09874
Protein Name
Poly [ADP-ribose] polymerase 1
Alternate Names
ADP-ribosyltransferase (NAD+ • poly (ADP-ribose) polymerase) • ADP-ribosyltransferase diphtheria toxin-like 1 • ADP-ribosyltransferase NAD(+) • ADPRT • ADPRT 1 • ADPRT1 • ARTD1 • NAD(+) ADP-ribosyltransferase 1 • pADPRT-1 • PARP • PARP-1 • poly (ADP-ribose) polymerase family, member 1 • poly ADP-ribose polymerase 1 • poly(ADP-ribose) synthetase • poly(ADP-ribosyl)transferase • polyADP-ribose synthase 1 • PPOL
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PARP1 immobilized on solid support.
The epitope recognized by IHC-00279 maps to a region between residue 50 and 100 of human poly (ADP-ribose) polymerase family, member 1 using the numbering given in entry NP_001609.1 (GeneID 142).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00279Open
Product DatasheetPDF · IHC-00279 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00279 · Lot 1 (current lot)Open
Citations / Publications
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