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Rabbit anti-Pat1b Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
Pat1b
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 425 and 475
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Country of Origin
USA

Additional Product Information

Pat1 homolog b (Pat1b) is an RNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Pat1b acts as a scaffold protein that connects deadenylation and decapping machinery. Pat1b is required for cytoplasmic mRNA processing body (P-body) assembly, and in cases of infection, it is required for translation and replication of hepatitis C virus (HCV) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q86TB9].

219988
PATL1
PAT1 homolog 1, processing body mRNA decay factor
Q86TB9
Protein PAT1 homolog 1

Alternate Names

hPat1b; Pat1b; PAT1-like protein 1; protein associated with topoisomerase II homolog 1; protein PAT1 homolog 1; protein PAT1 homolog b

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.