Rabbit anti-Pat1b Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Pat1 homolog b (Pat1b) is an RNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Pat1b acts as a scaffold protein that connects deadenylation and decapping machinery. Pat1b is required for cytoplasmic mRNA processing body (P-body) assembly, and in cases of infection, it is required for translation and replication of hepatitis C virus (HCV) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q86TB9].
Alternate Names
hPat1b; Pat1b; PAT1-like protein 1; protein associated with topoisomerase II homolog 1; protein PAT1 homolog 1; protein PAT1 homolog b
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.