Bethyl Laboratories
Rabbit anti-Pc2 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00668
TargetPc2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
Pc2 (polycomb 2 homolog) is also known as CBX4. Pc2 is an E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. It is a component of the Polycomb group (PcG) multiprotein complex, PRC1 (polycomb repressive complex), a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. The PcG-PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O00257].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetPc2
Antigen Species
Human
Gene ID8535
Gene Symbol
CBX4
Gene Name
chromobox 4
Uniprot IDO00257
Protein Name
E3 SUMO-protein ligase CBX4
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Pc2 immobilized on solid support.
The epitope recognized by IHC-00668 maps to a region between residue 150 and 200 of human polycomb 2 (chromobox homolog 4 ) using the numbering given in entry NP_003646.2 (GeneID 8535).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00668Open
Product DatasheetPDF · IHC-00668 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00668 · Lot 1 (current lot)Open
Citations / Publications
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