Bethyl Laboratories
Rabbit anti-PCNA Antibody Affinity Purified
Primary
Catalog #A300-277A
TargetPCNA
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IHC-IF, IP, WB
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Product Description
PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 211 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPCNA
Antigen Species
Human
Gene ID5111
Gene Symbol
PCNA
Gene Name
proliferating cell nuclear antigen
Uniprot IDP12004
Protein Name
Proliferating cell nuclear antigen
Alternate Names
Product Information
Applications
IHC, IHC-IF, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PCNA immobilized on solid support.
The epitope recognized by A300-277A maps to a region between residue 225 and the C-terminus (residue 261) of human proliferating cell nuclear antigen using the numbering given in SwissProt entry P12004 (GeneID 5111).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:2000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:1000 - 1:5000
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:5000 - 1:20,000
Documents
Product DatasheetPDF · A300-277A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-277A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-277AOpen
Citations / Publications
Services
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