Bethyl Laboratories
Rabbit anti-PDZ-GEF1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00594
TargetPDZ-GEF1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
PDZ-GEF1 is a guanine nucleotide exchange factor (GEF) for Rap GTPases. It contains one PDZ-signaling domain, a Ras-association (RA) domain, and a region related to a cyclic nucleotide binding domain (RCBD). PDZ-GEF1 functions to catalyze the exchange of GDP for GTP and activate Rap1A, Rap1B, and Rap2B GTPase activity.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1000 and 1050
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetPDZ-GEF1
Antigen Species
Human
Gene ID9693
Gene Symbol
RAPGEF2
Gene Name
Rap guanine nucleotide exchange factor 2
Uniprot IDQ9Y4G8
Protein Name
Rap guanine nucleotide exchange factor 2
Alternate Names
CNrasGEF • cyclic nucleotide ras GEF • neural RAP guanine nucleotide exchange protein • nRap GEP • NRAPGEP • PDZ domain containing guanine nucleotide exchange factor (GEF) 1 • PDZ domain-containing guanine nucleotide exchange factor 1 • PDZGEF1 • PDZ-GEF1 • RA(Ras/Rap1A-associating)-GEF • RAGEF • RA-GEF • RA-GEF-1 • Rap guanine nucleotide exchange factor (GEF) 2 • rap guanine nucleotide exchange factor 2 • Rap-GEP • ras/Rap1-associating GEF-1
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PDZ-GEF1 immobilized on solid support.
The epitope recognized by IHC-00594 maps to a region between residue 975 and 1025 of human PDZ domain containing guanine nucleotide exchange factor (GEF) 1 using the numbering given in entry NP_055062.1 (GeneID 9693).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer or tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate Buffer pH 6.0 is recommended for FFPE tissue sections.
Documents
Safety Data SheetPDF · IHC-00594Open
Product DatasheetPDF · IHC-00594 · Lot 3 (current lot)Open
Product DatasheetPDF · IHC-00594 · Lot 3 (current lot)Open
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