Bethyl Laboratories
Rabbit anti-PDZ-RhoGEF Antibody Affinity Purified
Primary
Catalog #A301-952A
TargetPDZ-RhoGEF
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
PDZ-RhoGEF. (PDZ domain-containing Rho guanine nucleotide exchange factor) catalyzes the exchange of GDP for GTP on the GTPase, Rho, to activate downstream signaling pathways. It contains a PDZ [post synaptic density protein (PSD95), Drosophila dis large tumor suppressor (DIgA), and zonula occludens-1 protein (zo-1)] domain which is an 80-90 amino acid structural domain found in signaling proteins in bacteria, yeast, plants, and animals. PDZ-RhoGEF has been shown to be important to cell migration and the regulation of adhesion movement and trailing edge retraction.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1472 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetPDZ-RhoGEF
Antigen Species
Human
Gene ID9826
Gene Symbol
ARHGEF11
Gene Name
Rho guanine nucleotide exchange factor 11
Uniprot IDO15085
Protein Name
Rho guanine nucleotide exchange factor 11
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PDZ-RhoGEF immobilized on solid support.
The epitope recognized by A301-952A maps to a region between residue 1472 and 1522 of human PDZ-RhoGEF using the numbering given in entry NP_055599.1 (GeneID 9826).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-952A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-952A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-952AOpen
Citations / Publications
Services
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