Bethyl Laboratories
Rabbit anti-Pescadillo Antibody Affinity Purified
Primary
Catalog #A300-902A
TargetPescadillo
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
Pescadillo is a BRCT (BRCA1 C-terminal) domain protein and plays a role in DNA synthesis, ribosome genesis, cell proliferation, and transformation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 25 and 75
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPescadillo
Antigen Species
Human
Gene ID23481
Gene Symbol
PES1
Gene Name
pescadillo ribosomal biogenesis factor 1
Uniprot IDO00541
Protein Name
Pescadillo homolog
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Pescadillo immobilized on solid support.
The epitope recognized by A300-902A maps to a region between residue 25 and 75 of human Pescadillo using the numbering given in entry NP_055118.1 (GeneID 23481).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A300-902A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-902A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-902AOpen
Citations / Publications
Services
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