Rabbit anti-PEX14 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A303-086A maps to a region between residue 327 and 377 of human Peroxisomal Biogenesis Factor 14 using the numbering given in entry NP_004556.1 (GeneID 5195).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PEX14 immobilized on solid support.
The epitope recognized by A303-086A-T maps to a region between residue 327 and 377 of human Peroxisomal Biogenesis Factor 14 using the numbering given in entry NP_004556.1 (GeneID 5195).
Additional Product Information
Peroxisomal biogenesis factor 14 (PEX14) is an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome. [taken from NCBI Entrez Gene (Gene ID: 5195)].
Alternate Names
dJ734G22.2; NAPP2; NF-E2 associated polypeptide 2; PBD13A; peroxin-14; peroxisomal membrane anchor protein PEX14; peroxisomal membrane anchor protein Pex14p; peroxisomal membrane protein PEX14; Pex14p; PTS1 receptor docking protein; PTS1 receptor-docking protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.