Bethyl Laboratories
Rabbit anti-PF1 Antibody Affinity Purified
Primary
Catalog #A301-646A
TargetPF1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
PHD factor 1 (PF1) was identified as a protein that interacts with mSin3A-histone deacetylase corepressor, a multiprotein complex that mediates transcriptional repression. PF1 is a plant homeodomain zinc finger protein that also bears a forkhead-associated (FHA) domain. PF1 appears to facilitate the recruitment of the mSin3A complex to DNA for transcriptional repression. It has also been shown to interact with the histone deacetylase containing corepressor transducin-like enhancer of split (TLE) and may play a role in bridging the mSin3A and TLE transcriptional networks.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 550 and 600
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPF1
Antigen Species
Human
Gene ID57649
Gene Symbol
PHF12
Gene Name
PHD finger protein 12
Uniprot IDQ96QT6
Protein Name
PHD finger protein 12
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PF1 immobilized on solid support.
The epitope recognized by A301-646A maps to a region between residue 550 and 600 of human PHD factor 1 using the numbering given in entry NP_001028733.1 (GeneID 57649).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-646A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-646A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-646AOpen
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