/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-PHF6 Antibody
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Primary Antibodies  

Rabbit anti-PHF6 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (4)
Target:

PHF6

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 150 and 200

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Bovine

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to PHF6 immobilized on solid support.

The epitope recognized by A301-451A maps to a region between residue 125 and 175 of human PHD finger protein 6 using the numbering given in entry NP_115834.1 (GeneID 84295).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to PHF6 immobilized on solid support.

The epitope recognized by A301-451A-T maps to a region between residue 125 and 175 of human PHD finger protein 6 using the numbering given in entry NP_115834.1 (GeneID 84295).

Country of Origin

USA

Additional Product Information

PHF6 a member of the PHD (plant homeodomain)-like zinc-finger family of proteins and is suggested to function as an important transcription factor. PHF6 is ubiquitously expressed and localizes to the cell nucleus with prominent expression in the nucleolus. Expression in the nucleolus indicates an importance to ribosome biogenesis. Mutations in PHF6 are the cause of Borgeson-Forssman-Lehman syndrome (BFLS), an X-linked syndrome characterized by mental retardation, hypogonadism, hypometabolism, obesity, gynecomastia, and several forms of facial anomalies.

84295

PHF6

PHD finger protein 6

Q8IWS0

PHD finger protein 6

Alternate Names

BFLS; BORJ; CENP-31; centromere protein 31; PHD finger protein 6; PHD-like zinc finger protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.