Bethyl Laboratories
Rabbit anti-PHF8 Antibody Affinity Purified
Primary
Catalog #A301-772A
TargetPHF8
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationChIP, IHC, IP, WB
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Product Description
PHF8 (PHD finger protein 8) is a member of the jumonji family of proteins and contains a jumonji C (JmjC) domain. The JmjC proteins are predicted to be metalloenzymes that play a role a chromatin remodeling and histone demethylation. PHF8 also bears a PHD (plant homeodomain)- type zinc-finger, a domain also found to be involved in chromatin remodeling and transcriptional regulation. Truncating mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 675 and 725
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetPHF8
Antigen Species
Human
Gene ID23133
Gene Symbol
PHF8
Gene Name
PHD finger protein 8
Uniprot IDQ9UPP1
Protein Name
Histone lysine demethylase PHF8
Alternate Names
Product Information
Applications
ChIP, IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PHF8 immobilized on solid support.
The epitope recognized by A301-772A maps to a region between residue 650 and 700 of human PHD finger protein 8 using the numbering given in entry CAI41576.1 (GeneID 23133).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
ChIP-SeqChIP-Seq 10 µg as per Ram O et al., Cell. 2011 Dec 23;147(7):1628-39. Previous lots of this antibody have performed in this application.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-772A · Lot 4 (current lot)Open
Product DatasheetPDF · A301-772A · Lot 4 (current lot)Open
Safety Data SheetPDF · A301-772AOpen
Citations / Publications
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