Bethyl Laboratories
Rabbit anti-Phospho BID (S78) Antibody Affinity Purified
Primary
Catalog #A300-528A
TargetBID Phospho (S78)
HostRabbit
ClonalityPolyclonal
ReactivityMouse
ApplicationWB
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Product Description
BID (BH3-interacting domain death agonist) can mediate both apoptotic and pro-survival signals in response to DNA damage. BID is phosphorylated by ATM in response to DNA damage and may play a role in the cell’s decision of whether to live or die based on the extent of damage.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Surrounding serine 78
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
500 µg/ml
Target Identity
TargetBID Phospho (S78)
Antigen Species
Mouse
Gene ID12122
Gene Symbol
Bid
Gene Name
BH3 interacting domain death agonist
Uniprot IDP70444
Protein Name
BH3-interacting domain death agonist
Alternate Names
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BID phosphorylated on Serine 78 immobilized on solid support.
The epitope recognized by A300-528A maps to phosphorylated Serine 78 and surrounding residues of mouse BH3 interacting domain death agonist using the numbering given in entry NP_031570.2 (GeneID 12122).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:250 - 1:1000
Documents
Product DatasheetPDF · A300-528A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-528A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-528AOpen
Citations / Publications
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