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Rabbit anti-Phospho Histone H3 (S10) Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

Histone H3 Phospho (S10)

Reactivity:

Human

,

Mouse

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

surrounding serine 10

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Chicken,

Pig,

Rabbit,

X. laevis,

X. tropicalis,

Zebrafish,

Bovine,

Rat,

D. melanogaster

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to Phospho Histone H3 (Ser10) immobilized on solid support. Immunogen for A301-844A was a synthetic phosphorylated peptide, which represented a portion of human Histone H3 surrounding serine 10 according to the numbering given in entry NP_003520.1 (GeneID 8350).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to Phospho Histone H3 (Ser10) immobilized on solid support.

Immunogen for A301-844A-T was a synthetic phosphorylated peptide, which represented a portion of human Histone H3 surrounding serine 10 according to the numbering given in entry NP_003520.1 (GeneID 8350).

Additional Product Information

Histone H3 is one of four histone proteins that assemble into an octamer to form the nucleosome core and serve to spool and compact DNA into chromatin. Histones are subject to a complex set of post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination, sumoylation, citrullination, and ADP ribosylation) that are important to the regulation of gene transcription, DNA replication, and DNA repair.

8350

HIST1H3A

histone cluster 1 H3 family member a

P68431

Histone H3.1

Alternate Names

H3 histone family, member A; H3/A; H3FA; histone 1, H3a; histone cluster 1, H3a; histone H3.1; histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).