Bethyl Laboratories
Rabbit anti-Phospho MCM2 (S53) Antibody Affinity Purified
Primary
Catalog #A300-756A
TargetMCM2 Phospho (S53)
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, WB
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Product Description
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM2 interacts with the core MCM complex (MCM4, 6, and 7) to regulate its helicase activity.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Surrounding serine 53
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetMCM2 Phospho (S53)
Antigen Species
Human
Gene ID4171
Gene Symbol
MCM2
Gene Name
minichromosome maintenance complex component 2
Uniprot IDP49736
Protein Name
DNA replication licensing factor MCM2
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Phospho MCM2-S53 immobilized on solid support.
Immunogen was a synthetic phosphorylated peptide, which represented a portion of human minichromosomal maintenance deficient 2 surrounding Serine 53 according to the numbering given in entry NP_004517.2 (GeneID 4171).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000 - 1:2500
Documents
Product DatasheetPDF · A300-756A · Lot 4 (current lot)Open
Product DatasheetPDF · A300-756A · Lot 4 (current lot)Open
Safety Data SheetPDF · A300-756AOpen
Citations / Publications
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