Bethyl Laboratories
Rabbit anti-Phospho XRCC1 (S461) Antibody Affinity Purified
Primary
Catalog #A300-147A
TargetXRCC1 Phospho (S461)
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
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Product Description
X-ray repair cross-complementing protein 1 (XRCC1) has been identified as a gene that partially rescues a DNA repair defect and therefore plays a role in efficient repair of single-stranded breaks in DNA damage caused by ionizing radiation. XRCC1 interacts with human polynucleotide kinase, DNA polymerase-beta, and DNA ligase III in a multiprotein complex to execute the function of DNA repair.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Surrounding serine 461
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetXRCC1 Phospho (S461)
Antigen Species
Human
Gene ID7515
Gene Symbol
XRCC1
Gene Name
X-ray repair cross complementing 1
Uniprot IDP18887
Protein Name
DNA repair protein XRCC1
Product Information
Applications
IHC, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to phosphorylation at Ser 461 of XRCC1 immobilized on solid support.
The epitope recognized by A300-147A maps to a region of surrounding residue Ser 416 of human X-ray Repair Complementing protein 1 using the numbering given in SwissProt entry P18887 (GeneID 7515).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A300-147A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-147A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-147AOpen
Citations / Publications
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