Rabbit anti-Phospho ZAP70 (Y319) Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-007A-T maps to a region including tyrosine 319 (when it is phosphorylated) of human zeta-chain (TCR) associated protein kinase 70kDa using the numbering given in entry NP_001070.2 (GeneID 7535).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Phospho ZAP70 (Y319) immobilized on solid support.
The epitope recognized by A302-007A maps to a region including tyrosine 319 (when it is phosphorylated) of human zeta-chain (TCR) associated protein kinase 70kDa using the numbering given in entry NP_001070.2 (GeneID 7535).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
ZAP-70 (zeta associated protein 70) is a tyrosine kinase of the Src family of tyrosine kinases. It was initially identified as a partner of the zeta chain of the CD3 complex associated to the T-cell receptor on T-lymphocytes. ZAP-70 shares homology with spleen-tyrosine kinase (Syk), a kinase involved in B-cell activation. Both ZAP-70 and Syk have been shown to be crucial to B- and T-lymphocyte development. In T-cells ZAP-70 functions to transduce the signaling cascade activated by T-cell receptor engagement and activation.
Alternate Names
70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; IMD48; SRK; STCD; STD; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP-70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.