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Rabbit anti-PHPT1 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
PHPT1
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 75 and 125
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Mouse, Human
Presumed Reactivity
Orangutan
Antigen Species
Human
Stock Concentration
40 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to PHPT1 immobilized on solid support.

The epitope recognized by A305-887A-T maps to a region between residue 75 and 125 of human 14 kDa phosphohistidine phosphatase using the numbering given in entry Q9NRX4.1 (GeneID 29085).

Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

PHPT1 is enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. [taken from NCBI Entrez Gene (Gene ID: 29085)].

29085

PHPT1

phosphohistidine phosphatase 1

Q9NRX4

14 kDa phosphohistidine phosphatase

Alternate Names

14 kDa phosphohistidine phosphatase, CGI-202, epididymis secretory sperm binding protein Li 132P, HEL-S-132P, HSPC141, Phosphohistidine phosphatase 1, PHP, PHP14, PHPT1, protein histidine phosphatase, protein janus-A homolog, sex-regulated protein janus-a

Applications

Western blot of lysates performed using standard western blot reagents and 4-12% SDS-PAGE.