Bethyl Laboratories

Rabbit anti-PIMT IHC Antibody Affinity Purified

Primary
Catalog #IHC-00467
TargetPIMT
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
Detection of human PIMT by immunohistochemistry.
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Product Description

PIMT, also known as TGS1, was originally identified in yeast as a protein involved in RNA cap trimethylation and the transfer of methyl from S-adenosylmethionine to m7GTP and m7GDP.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetPIMT
Antigen Species
Human
Gene ID96764
Gene Symbol
TGS1
Gene Name
trimethylguanosine synthase 1
Uniprot IDQ96RS0
Protein Name
Trimethylguanosine synthase
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to PIMT immobilized on solid support.

The epitope recognized by IHC-00467 maps to a region between residue 150 and 200 of human PRIP-Interacting Protein with Methyltransferase Domain (Nuclear Receptor Coactivator 6 Interacting Protein) using the numbering given in entry NP_079107.5 (GeneID 96764).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Epitope exposure is recommended.

Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500

Documents

Safety Data SheetPDF · IHC-00467Open
Product DatasheetPDF · IHC-00467 · Lot 2 (current lot)Open
Product DatasheetPDF · IHC-00467 · Lot 2 (current lot)Open

Citations / Publications

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