Bethyl Laboratories
Rabbit anti-PKC-alpha Antibody Affinity Purified
Primary
Catalog #A302-445A
TargetPKC-alpha
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Protein kinase C alpha (PKC-alpha) is a member of the PKC family of serine-threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. PKC-alpha has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control [taken from NCBI Entrez Gene (GeneID: 5578)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 300 and 350
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPKC-alpha
Antigen Species
Human
Gene ID5578
Gene Symbol
PRKCA
Gene Name
protein kinase C alpha
Uniprot IDP17252
Protein Name
Protein kinase C alpha type
Alternate Names
AAG6 • aging-associated gene 6 • PKCA • PKC-A • PKCalpha • PKC-alpha • PKCI+/- • PRKACA • protein kinase C alpha type
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PKC-alpha immobilized on solid support.
The epitope recognized by A302-445A maps to a region between residue 300 and 350 of human protein kinase C, alpha using the numbering given in entry NP_002728.1 (GeneID 5578).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-445A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-445A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-445AOpen
Citations / Publications
Services
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