Bethyl Laboratories
Rabbit anti-PML IHC Antibody Affinity Purified
Primary
Catalog #IHC-00241
TargetPML
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
PML is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. PML is a multifunctional protein involved in activities such as tumor growth suppression, hematopoietic differentiation, gene transcription, apoptotic induction, and viral immunity. PML is often involved in chromosomal translocations with the retinoic acid receptor alpha gene. PML-retinoic acid receptor translocations are associated with acute promyelocytic leukemia.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 400 and 450
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetPML
Antigen Species
Human
Gene ID5371
Gene Symbol
PML
Gene Name
promyelocytic leukemia
Uniprot IDP29590
Protein Name
Protein PML
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PML immobilized on solid support.
The epitope recognized by IHC-00241 maps to a region between residue 375 and 425 of promyelocytic leukemia using the numbering given in entry NP_150241.2 (GeneID 5371).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00241Open
Product DatasheetPDF · IHC-00241 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00241 · Lot 1 (current lot)Open
Services
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