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Rabbit anti-PMS2 Recombinant Monoclonal Antibody [BLR142H]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

PMS2

Reactivity:

Human

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR142H

Format:

Whole IgG

Immunogen:

between 550 and 600

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (10 blots)

20 µl (2 blots)

100 µg (BSA-Free)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
10 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a peptide representing a region between residue 550 and 600 of human mismatch repair endonuclease PMS2 using the numbering given in entry NP_000526.2 (Gene ID 5395).

Additional Product Information

PMS2 is an integral component of the DNA mismatch repair (MMR) system that functions to recognize aberrant structures and signals for the repair of single base mismatches or loops in replicated DNA. PMS2 can heterodimerize with DNA mismatch repair protein MLH1 to form the MutL-alpha heterodimer. [taken from NCBI Entrez Gene (Gene ID: 5395)].

5395

PMS2

PMS1 homolog 2, mismatch repair system component

P54278

Mismatch repair endonuclease PMS2

Alternate Names

DNA mismatch repair protein PMS2; HNPCC4; mismatch repair endonuclease PMS2; MLH4; PMS1 homolog 2, mismatch repair protein; PMS1 protein homolog 2; PMS2 postmeiotic segregation increased 2; PMS2CL; PMSL2; postmeiotic segregation increased 2 nirs variant 6

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.