Bethyl Laboratories
Rabbit anti-Pol Lambda Antibody Affinity Purified
Primary
Catalog #A301-641A
TargetPol Lambda
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP
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Product Description
DNA polymerase lambda (pol lambda) is a DNA polymerase involved in base excision repair (BER). Pol lambda possesses terminal transferase and 5’-deoxyribose-5-phosphate lyase activity. These activities have been found to be coordinated by PCNA and replication protein A.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 525 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPol Lambda
Antigen Species
Human
Gene ID27343
Gene Symbol
POLL
Gene Name
DNA polymerase lambda
Uniprot IDQ9UGP5
Protein Name
DNA polymerase lambda
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Pol Lambda immobilized on solid support.
The epitope recognized by A301-641A maps to a region between residue 525 and 575 of human polymerase (DNA directed), lambda using the numbering given in entry NP_037406.1 (GeneID 27343).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-641A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-641A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-641AOpen
Citations / Publications
Services
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