Bethyl Laboratories
Rabbit anti-POLR3D IHC Antibody Affinity Purified
Primary
Catalog #IHC-00662
TargetPOLR3D
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
RNA polymerase III subunit D (POLR3D) is one of 17 subunits of the RNA polymerase III (PolIII) which synthesizes small RNAs, such as 5S rRNA and tRNA.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 175 and 225
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetPOLR3D
Antigen Species
Human
Gene ID661
Gene Symbol
POLR3D
Gene Name
RNA polymerase III subunit D
Uniprot IDP05423
Protein Name
DNA-directed RNA polymerase III subunit RPC4
Alternate Names
BN51 (BHK21) temperature sensitivity complementing • BN51T • DNA-directed RNA polymerase III 47 kDa polypeptide • DNA-directed RNA polymerase III subunit D • DNA-directed RNA polymerase III subunit RPC4 • polymerase (RNA) III (DNA directed) polypeptide D, 44kDa • polymerase (RNA) III subunit D • Protein BN51 • RNA polymerase III 47 kDa subunit • RNA polymerase III subunit C4 • RPC4 • RPC53 • RPC53 homolog • temperature sensitive complementation, cell cycle specific, tsBN51 • TSBN51
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to POLR3D immobilized on solid support.
The epitope recognized by IHC-00662 maps to a region between residue 175 and 225 of human DNA-directed RNA polymerase III subunit D using the numbering given in entry NP_001713.2 (GeneID 661).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00662Open
Product DatasheetPDF · IHC-00662 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00662 · Lot 1 (current lot)Open
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