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Rabbit anti-PP2A Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
PP2A
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Rat
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to PP2A immobilized on solid support.

The epitope recognized by A300-731A maps to a region between residue 1 and 50 of human Protein Phosphatase 2A (PPP2CB, Protein Phosphatase 2, Catalytic Subunit, Beta isoform) using the numbering given in entry NP_004147.1 (GeneID 5516). The region is highly conserved with PPP2CA (NP_002706.1; GeneID: 5515)

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to PP2A immobilized on solid support.

The epitope recognized by A300-731A-T maps to a region between residue 1 and 50 of human Protein Phosphatase 2A (PPP2CB, Protein Phosphatase 2, Catalytic Subunit, Beta isoform) using the numbering given in entry NP_004147.1 (GeneID 5516). The region is highly conserved with PPP2CA (NP_002706.1; GeneID: 5515)
Country of Origin
USA

Additional Product Information

PP2A is a major serine/threonine phosphatase that is implicated in the negative control of cell cycle progression. PP2A encodes the beta isoform of the catalytic subunit (PP2CB). Chk2 has been shown to be negatively regulated and dephosphorylated by PP2A. Several proteins have been shown to associate with PP2A and regulate its activity such as eRF1, Hox11, CK2, and Igbp1/alpha4. Rapamycin has been shown to dissociate PP2A from alpha-4 and inhibit proliferation. This result suggests an alternative positive regulatory role of PP2A in cell proliferation.

5516
PPP2CB
protein phosphatase 2 catalytic subunit beta
P62714
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform

Alternate Names

PP2Abeta; PP2A-beta; PP2CB; protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform; protein phosphatase 2, catalytic subunit, beta isoform; protein phosphatase 2, catalytic subunit, beta isozyme; protein phosphatase 2A catalytic subunit, beta isoform; protein phosphatase type 2A catalytic subunit; serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform; serine/threonine-protein phosphatase 2A catalytic subunit beta isoform; testicular tissue protein Li 146

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).