/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-PPAN Antibody
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Primary Antibodies  

Rabbit anti-PPAN Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

PPAN

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 375 and 425

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Peter Pan homolog (PPAN) is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells [taken from NCBI Entrez Gene (Gene ID: 56342)].

56342

PPAN

peter pan homolog

Q9NQ55

Suppressor of SWI4 1 homolog

Alternate Names

brix domain-containing protein 3; BXDC3; homolog of S. cerevisiae SSF1; Peter Pan homolog; second-step splicing factor 1; SSF; SSF1; SSF-1; SSF2; suppressor of sterile four 1; suppressor of SWI4 1 homolog

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.