Bethyl Laboratories

Rabbit anti-PPP1CA Antibody Affinity Purified

Primary
Catalog #A300-904A
TargetPPP1CA
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, WB
Detection of human PPP1CA by immunohistochemistry.
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Product Description

Protein phosphatase 1 (PP1) is a major eukaryotic serine/threonine phosphatase that is involved in a multitude of cellular functions. PP1 is a holoenzyme that consists of the PP1 catalytic subunit (PP1c) that associates with and is regulated by over 50 regulatory subunits. There are several gene and alternative splice products of the catalytic subunit PP1c. PPP1CA encodes the PP1 alpha catalytic subunit. In addition to PP1 alpha, there is PP1 beta (PPP1CB), and two splice variants of PP1 gamma (PPP1CC). The mutually exclusive interaction between the various catalytic and regulatory subunits allows the PP1 enzyme to be involved in a variety of functions which include cell division, glycogen metabolism, muscle contractility, and protein synthesis. serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PPP1A, PP-1A, MGC1674, MGC15877.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 280 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPPP1CA
Antigen Species
Human
Gene ID5499
Gene Symbol
PPP1CA
Gene Name
protein phosphatase 1 catalytic subunit alpha
Uniprot IDP62136
Protein Name
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Product Information
Applications
IHC, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to PPP1CA immobilized on solid support.

The epitope recognized by A300-904A maps to a region between residue 280 and the C-terminus (residue 330) of human Protein Phosphatase 1, Catalytic Subunit, Alpha Isoform using the numbering given in entry NP_002699.1 (GeneID 5499).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Immunohistochemistry (IHC)1:500 to 1:2000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000

Documents

Product DatasheetPDF · A300-904A · Lot 3 (current lot)Open
Product DatasheetPDF · A300-904A · Lot 3 (current lot)Open
Safety Data SheetPDF · A300-904AOpen

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