Bethyl Laboratories

Rabbit anti-PPP1CB IHC Antibody Affinity Purified

Primary
Catalog #IHC-00484
TargetPPP1CB
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
Detection of human PPP1CB by immunohistochemistry.
Image 1 of 1
local_shipping

Order within 1 hours and 16 minutes for shipment by Tuesday, November 3

Product Description

Protein phosphatase 1 (PP1) is a major eukaryotic serine/threonine phosphatase that is involved in a multitude of cellular functions. PP1 is a holoenzyme that consists of the PP1 catalytic subunit (PP1c) that associates with and is regulated by over 50 regulatory subunits. There are several gene and alternative splice products of the catalytic subunit PP1c. PPP1CB encodes the PP1 beta catalytic subunit. In addition to PP1 beta, there are two splice variants of PP1 alpha (PPP1CA), and PP1 gamma (PPP1CC). The mutually exclusive interaction between the various catalytic and regulatory subunits allows the PP1 enzyme to be involved in a variety of functions which include cell division, glycogen metabolism, muscle contractility, and protein synthesis. serine/threonine-protein phosphatase PP1-beta catalytic subunit, PP-1B, PPP1CD, MGC3672.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 277 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetPPP1CB
Antigen Species
Human
Gene ID5500
Gene Symbol
PPP1CB
Gene Name
protein phosphatase 1 catalytic subunit beta
Uniprot IDP62140
Protein Name
Serine/threonine-protein phosphatase PP1-beta catalytic subunit
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to PPP1CB immobilized on solid support.

The epitope recognized by IHC-00484 maps to a region between residue 280 and the C-terminus (residue 327) of human Protein Phosphatase 1, Catalytic Subunit, Beta Isoform using the numbering given in entry NP_996759.1 (GeneID 5500).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Epitope exposure is recommended.

Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500

Documents

Safety Data SheetPDF · IHC-00484Open
Product DatasheetPDF · IHC-00484 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00484 · Lot 1 (current lot)Open
Using this product in your research?Published applications help other scientists evaluate reagents and accelerate assay development.Upload Your Publication

Related Products

Services

Resources