Bethyl Laboratories

Rabbit anti-PPP1CC IHC Antibody Affinity Purified

Primary
Catalog #IHC-00485
TargetPPP1CC
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
Detection of human PPP1CC by immunohistochemistry.
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Product Description

Protein phosphatase 1 (PP1) is a major eukaryotic serine/threonine phosphatase that is involved in a multitude of cellular functions. PP1 is a holoenzyme that consists of the PP1 catalytic subunit (PP1c) that associates with and is regulated by over 50 regulatory subunits. There are several gene and alternative splice products of the catalytic subunit PP1c. PPP1CC encodes the PP1 gamma catalytic subunit. In addition to PP1 gamma, there are two splice variants of PP1 alpha (PPP1CA), and a PP1 beta (PPP1CB) form. The mutually exclusive interaction between the various catalytic and regulatory subunits allows the PP1 enzyme to be involved in a variety of functions which include cell division, glycogen metabolism, muscle contractility, and protein synthesis. serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP-1G, PPP1G.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 273 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetPPP1CC
Antigen Species
Human
Gene ID5501
Gene Symbol
PPP1CC
Gene Name
protein phosphatase 1 catalytic subunit gamma
Uniprot IDP36873
Protein Name
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to PPP1CC immobilized on solid support.

The epitope recognized by IHC-00485 maps to a region between residue 280 and the C-terminus (residue 323) of human Protein Phosphatase 1, Catalytic Subunit, Gamma Isoform using the numbering given in entry NP_002701.1 (GeneID 5501).



Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Epitope exposure is recommended.

Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500

Documents

Safety Data SheetPDF · IHC-00485Open
Product DatasheetPDF · IHC-00485 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00485 · Lot 1 (current lot)Open
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