Bethyl Laboratories
Rabbit anti-PPP2R5D Antibody Affinity Purified
Primary
Catalog #A301-100A
TargetPPP2R5D
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
PP2A is a major serine/threonine phosphatase that is implicated in the negative control of cell cycle progression. It is a trimeric holoenzyme that is comprised of a catalytic, structural, and regulatory subunit. PPP2R5D is a regulatory subunit of PP2A. The regulatory subunits of PP2A are grouped into 3 families: B/PR55, B’/PR61, and B’’/PR72. PPP2R5D encodes the delta isoform of the B’ subunit, B56. PPP2R5D may function to localize PP2A to the nucleus. serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform, PP2A B subunit B’ delta isoform, PP2A B subunit B56 delta isoform, PP2A B subunit PR61 delta isoform, PP2A B subunit R5 delta isoform, B56D, MGC2134, MGC8949.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 552 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetPPP2R5D
Antigen Species
Human
Gene ID5528
Gene Symbol
PPP2R5D
Gene Name
protein phosphatase 2 regulatory subunit B'delta
Uniprot IDQ14738
Protein Name
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
Alternate Names
B56D • B56delta • MRD35 • PP2A B subunit isoform B56-delta • PP2A B subunit isoform B'-delta • PP2A B subunit isoform PR61-delta • PP2A B subunit isoform R5-delta • PP2A, B subunit, B' delta isoform • PP2A, B subunit, B56 delta isoform • PP2A, B subunit, PR61 delta isoform • PP2A, B subunit, R5 delta isoform • protein phosphatase 2, regulatory subunit B (B56), delta isoform • Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, delta isoform • serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PPP2R5D immobilized on solid support.
The epitope recognized by A301-100A maps to a region between residue 552 and 602 of human serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta using the numbering given in entry NP_006236.1 (GeneID 5528).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-100A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-100A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-100AOpen
Citations / Publications
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